Wednesday, May 4, 2011

Suture materials

  • Natural synthetic
  • Absorbable non absorbable
  • Braided non braided

Sutures (also known as stitches) are divided into two kinds – those which are absorbable and will break down harmlessly in the body over time without intervention, and those which are non-absorbable and must be manually removed if they are not left indefinitely. The type of suture used varies on the operation, with the major criteria being the demands of the location and environment.

· Sutures to be placed internally would require re-opening if they were to be removed. Sutures which lie on the exterior of the body can be removed within minutes, and without re-opening the wound. As a result, absorbable sutures are often used internally; non-absorbable externally.

· Sutures to be placed in a stressful environment, for example the heart (constant pressure and movement) or the bladder (adverse chemical presence) may require specialized or stronger materials to perform their role; usually such sutures are either specially treated, or made of special materials, and are often non-absorbable to reduce the risk of premature degradation.

Absorbable sutures

Absorbable sutures are made of materials which are broken down in tissue after a given period of time, which depending on the material can be from ten days to eight weeks. They are used therefore in many of the internal tissues of the body. In most cases, three weeks is sufficient for the wound to close firmly. The suture is not needed any more, and the fact that it disappears is an advantage, as there is no foreign material left inside the body and no need for the patient to have the sutures removed.

Absorbable sutures were originally made of the intestines of sheep, the so called catgut. The manufacturing process was similar to that of natural musical strings for violins and guitar, and also of natural strings for tennis racquets. The inventor, a 10th century surgeon named al-Zahrawi reportedly discovered the dissolving nature of catgut when his lute's strings were eaten by a monkey. Today, gut sutures are made of specially prepared beef and sheep intestine, and may be untreated (plain gut), tanned with chromium salts to increase their persistence in the body (chromic gut), or heat-treated to give more rapid absorption (fast gut). However, the majority of absorbable sutures are now made of synthetic polymer fibers, which may be braided or monofilament; these offer numerous advantages over gut sutures, notably ease of handling, low cost, low tissue reaction, consistent performance and guaranteed non-toxicity. In Europe and Japan, gut sutures have been banned due to concerns over bovine spongiform encephalopathy (mad-cow disease), although the herds from which gut is harvested are certified BSE-free. Each major suture manufacturer has its own proprietary formulations for its brands of synthetic absorbable sutures; various blends of polyglycolic acid (Biovek for example), polylactic acid or caprolactone are common.

Occasionally, absorbable sutures can cause inflammation and be rejected by the body rather than absorbed.

Non-absorbable sutures

Nonabsorbable sutures are made of materials which are not metabolized by the body, and are used therefore either on skin wound closure, where the sutures can be removed after a few weeks, or in some inner tissues in which absorbable sutures are not adequate. This is the case, for example, in the heart and in blood vessels, whose rhythmic movement requires a suture which stays longer than three weeks, to give the wound enough time to close. Other organs, like the bladder, contain fluids which make absorbable sutures disappear in only a few days, too early for the wound to heal. Inflammation caused by the foreign protein in some absorbable sutures can amplify scarring, so if other types of suture are less antigenic (ie, do not provoke as much of an immune response) it would represent a way to reduce scarring.

There are several materials used for nonabsorbable sutures. The most common is a natural fiber, silk, which undergoes a special manufacturing process to make it adequate for its use in surgery. Other nonabsorbable sutures are made of artificial fibers, like polypropylene, polyester or nylon; these may or may not have coatings to enhance their performance characteristics. Finally, stainless steel wires are commonly used in orthopedic surgery and for sternal closure in cardiac surgery.

Glasgow coma scale

Glasgow Coma Scale or GCS, sometimes also known as the Glasgow Coma Score is a neurological scale which aims to give a reliable, objective way of recording the conscious state of a person, for initial as well as continuing assessment. A patient is assessed against the criteria of the scale, and the resulting points give a patient score between 3 (indicating deep unconsciousness) and either 14 (original scale) or 15 (the more widely used modified or revised scale).

GCS was initially used to assess level of consciousness after head injury, and the scale is now used by first aid, EMS and doctors as being applicable to all acute medical and trauma patients. In hospitals it is also used in chronic patient monitoring, in for instance, intensive care.

Eye Opening

E

spontaneous

4

to speech

3

to pain

2

no response

1

Best Motor Response

M

To Verbal Command:

obeys

6

To Painful Stimulus:

localizes pain

5

flexion-withdrawal

4

flexion-abnormal

3

extension

2

no response

1

Best Verbal Response

V

oriented and converses

5

disoriented and converses

4

inappropriate words

3

incomprehensible sounds

2

no response

1

E + M + V = 3 to 15

  • 90% less than or equal to 8 are in coma
  • Greater than or equal to 9 not in coma
  • 8 is the critical score
  • Less than or equal to 8 at 6 hours - 50% die
  • 9-11 = moderate severity
  • Greater than or equal to 12 = minor injury

Tumour Markers


Oncogenesis

Human cancer development is a result of a genetic disease

protoncogenes

Tumour suppressor genes.

Regulatory expression of these genes can be seen in the normal cells and protein products of these genes are fundamental for normal cell function.

Examples:

Oncogenes: Ras, Myc, EGFR,c-erbB1,2,3.

Tumour suppressor genes: p53, Rb genes

Carcinogenesis

Multifactorial etiology.

The tumour suppressor genes or oncogenes would be altered in the presence of carcinogens:

Chemical carcinogens

Physical agents

Ionizing radiation

Viral agents

Others

Four common types of genetic changes

Deletion : Loss of tumour suppressor genes

Mutation : p53, Ras

Inversion : Relocate the the oncogenes in

Translocation to DNA areas which will leads inappropriate transcription.

Subsequent accumulation of such growth promoting genetic defects forms the basis of current multi step process of tumourigenesis.

This multi step process includes.

Initiation.

Promotion.

Tumour progression.

Many of the known oncogenes and oncosupressor genes help control mitosis and apoptosis.

Lack of this control would leads to development of malignancy.

Genetic disease.

Tumour marker

Substance or group of substance produced by the tumour

Which can be used as a indicator to detect the presence of the tumour.

A tumour marker should be indicative of :

Tumour susceptibility of the patient.

Severity or virulence of the tumour.

Prognosis of the disease.

Tumour burden (Including metastasis) .

Treatment response.

Types of tumour markers:

Histopathological markers.

Immuno markers.

Genetic markers.

Hormonal markers.

Protein products of tumours Carcino-embrionic antigen

Apoptosis related genes

Bcl2 family: Bcl2, Bcl XL , Bax , Bad.

Antimicrobial Prophylaxis


GENERAL PRINCIPLES

1.ANTI MICROBIALS CANNOT ELIMINATE ALL MICRO ORGANISMS IN THE HOST AND CANNOT PREVENT ALL TYPES OF INFECTION. HENCE PROPHYLAXIS SHOULD BE DIRECTED AGAINST A SPECIFIC PATHOGEN OR USED TO PREVENT INFECTION AT A SPECIFIC SITE

2.SHORTER THE DURATION OF PROPHYLAXIS THE LARGER THE RANGE OF PATHOGENS AFFECTED. FOR PREVENTION OF ALL INFECTIONS IN LEUCOPOENIAS BROAD SPECTRUM ANTI MICROBIALS à SHORT TERM LONG TERM USE--à RESISTANCE GROUP “A” STREP àLONG TERM USE.

3. PROPHYLAXIS MORE EFFECTIVE AGAINST PATHOGENS LESS LIKELY TO DEVELOP RESISTANCE Eg. Penicillin for Grp.”A” strepàeffective Not for gonococcus

4. DRUGS FOR THERAPY MAY NOT BE GOOD FOR PROPHYLAXIS

Eg.PENICILLIN IN MENINGOCOCCAL MENINGITIS NOT EFFECTIVE FOR PROPHYLAXIS

5. PROPHYLACTIC DRUGS SHOULD ONLY BE USED WHEN EFFICACY HAS BEEN DOCUMENTED COST, TOXICITY, SUPERINFECTION DEVELOPMENT OF RESISTANCE

SURGICAL PROPHYLAXIS

n A M MUST BE EFFFECTIVE AGAINST MAJORITY OF ORGANISMS CAUSING POST.OP. INFECTIONS

n START THERAPY 1-2HOURS BEFORE AND STOP 12-48 HOURS AFTER OP.

n A SINGLE PRE OP.DOSE GIVES MAXIMAL BENEFIT

n A M ACTIVITY MUST BE PRESENT IN SURGICAL WOUND BEFORE CLOSURE

n DRUGS

n CEFAZOLIN -àDRUG OF CHOICE First Generation >cephalosporin 0.5GM -2GM I.V.

n CEFEPIME 4TH GENERATION CEPHALOSPORIN MORE RESISTANCE TO HYDROLYSIS BY BETA LACTAMASE

Eg. ENTEROBACTER THAT MAY INACTIVATE 3RD GENERATION CEPH.

n DRUG COMBINATIONS

n INDICATIONS

n VERY ILL PATIENTS WITH INFECTIONS OF UNKNOWN ORIGIN Eg SEPTICAEMIA ANTI STAPH (NAFCILLIN-CLOXACILLIN0 WITH Gm –ve BACILLI (GENTAMICIN TOBRAMYCIN AMIKACIN) IMMUNOCOMPROMISED.

n DRUG COMBINATIONS MIXED INFECTIONS PERITONITIS

ANAEROBES– metronidazole, Gm +ve Bacteria- clindamycin COLIFORMS --aminoglycosides

PREVENT RESISTANCE Eg. T.B. ISONIAZID,ETHAMBUTOL,RIFAMPIN.

ENHANCE EFFECT OF SINGLE DRUG Eg. SULPHONAMIDE WITH TRIMETHOPRIM

PENICILLIN WITH AMINOGLYSOIDE--à ENTEROCOCCUS FAECALIS

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